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UID:20260309T224109EDT-8342pViTJv@132.216.98.100
DTSTAMP:20260310T024109Z
DESCRIPTION:Guy G. Poirier\nPh.D.\nGroupe de recherche sur les mécanismes d
 e poly(ADP-ribosyl)ation\n(Axe Cancer\, CHUQ - Pavillon CHUL) Chaire du Ca
 nada en\nProtéomique\nPlateforme protéomique du Centre de Génomique de Qué
 bec\n“Role of poly(ADP-ribose)-binding proteins in DNA damage\nsignalling 
 and repair”\nOne of the earliest step in the DNA damage signaling is the\n
 recruitment of poly(ADP-ribose) polymerase-1 (PARP-1) at the\nvicinity of 
 the DNA strand break (DSB). In presence of DNA lesions\,\nPARP-1 is rapidl
 y activated and signals the presence of damage by\nattaching ADP-ribose un
 its to chromatin-associated proteins.\nPoly(ADP-ribosyl)ation may function
  to organize the recruitment of\nrepair proteins to the DSB by noncovalent
  pADPr-binding\ninteractions. The central role of poly(ADP-ribose) (pADPr)
  in the\nrecruitment and assembly of multiprotein repair complexes\nstreng
 hten the role of DNA-dependent PARPs as regulators of genome\nmaintenance 
 pathways.\nIn spite of the importance of pADPr in the molecular basis of\n
 DNA damage response\, few pADPr-binding proteins and\npADPr-containing mul
 tiprotein complexes have been identified so\nfar. Our laboratory is presen
 tly engaged in proteome-wide\nidentification of proteins with affinity to 
 pADPr. In silico\nprediction of pADPr-binding proteins\, large-scale mass
 \nspectrometry-based proteome analysis and protein microarray\nanalysis of
  pADPr-binding proteins were used to establish a\ncomprehensive repertoire
  of pADPr-associated proteins. The\ninvolvement of putative pADPr-binding 
 proteins in DNA damage\nsignaling is presently under investigation using i
 n vivo laser\nmicroirradiation-induced DNA damage which permits to evaluat
 e the\npADPr-dependent recruitment of protein at sites of DNA damage. We\n
 are currently using Multiple Reaction Monitoring (MRM)\, one of the\nmost 
 selective and sensitive mass spectrometry scan function\, for\nthe quantit
 ative analysis of pADPr-containing protein complexes\ninvolved in DNA dama
 ge response and repair. In addition\, we perform\nSILAC analysis (Stable I
 sotope Labeling with Amino acids in Cell\nculture) which allows quantitati
 ve assessment of proteins levels in\nanti-pADPr immunoprecipitation extrac
 ts with correlation with pADPr\nlevels.\n \nSupported by grants from Canad
 ian Institutes of Health Research\n(CIHR)\, the National Institutes of Hea
 lth (NIH) and the Alberta\nCancer Board.\n\nMandatory for Graduate Student
 s\n
DTSTART:20110606T200000Z
DTEND:20110606T200000Z
LOCATION:McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3655 pr
 omenade Sir William Osler
SUMMARY:Biochemistry Seminar Dr. Guy G. Poirier
URL:/channels/event/biochemistry-seminar-dr-guy-g-poir
 ier-174413
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